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The antibody against APLF was raised in rabbit using the Synthesized non-phosphopeptide derived from Human APLF around the phosphorylation site of serine 116 (R-N-S(p)-Q-V). as the immunogen. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on ELISA, WB, IHC.
The antibody against APLF was raised in rabbit using the Synthesized non-phosphopeptide derived from Human APLF around the phosphorylation site of serine 116 (R-N-S(p)-Q-V). as the immunogen. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on ELISA, WB, IHC.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using APLF (Ab-116) antibody.
Western blot analysis of extracts from HT-29 cells, using APLF (Ab-116) antibody.2
Western blot analysis of extracts from COS7 cells (Lane 2), using APLF (Ab-116) antiobdy. The lane on the left is treated with systhesized peptide.3
| Cat.No | ADC-42511A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | APLF |
| Form | Liquid | Species Reactivity | Human, Mouse |
| Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) | Purification Method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Application | ELISA, IHC, WB | Storage | Upon receipt |
| Immunogen Description | Synthesized non-phosphopeptide derived from Human APLF around the phosphorylation site of serine 116 (R-N-S(p)-Q-V). | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q8IW19 |
Uniprot Id
Q8IW19
Target Species
Human
Target Name
APLF
Target Full Name
Aprataxin and PNK-like factor
Target Function
Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ).
Target Subcellular Location
Nucleus. Chromosome. Cytoplasm, cytosol.
Target Protein Families
APLF family
Target Synonyms
2010301N04Rik; AI452191; Aplf; APLF_HUMAN; Aprataxin and PNK-like factor; Apurinic-apyrimidinic endonuclease APLF; C2orf13; PNK and APTX like FHA protein; PNK and APTX-like FHA domain-containing protein; RGD1565557; XIP1; XRCC1 interacting protein 1; XRCC1-interacting protein 1
Target Background
Enables DNA-(apurinic or apyrimidinic site) endonuclease activity; nuclease activity; and nucleotide binding activity. Involved in double-strand break repair via nonhomologous end joining and single strand break repair. Acts upstream of or within positive regulation of DNA ligation. Located in nucleoplasm. Is active in site of double-strand break.
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