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The antibody against ygcB was raised in rabbit using the Recombinant Escherichia coli (strain K12) CAS3 protein as the immunogen. This antibody exists as a non-conjugated isotype IgG, Protein A/G purified. This antibody has been validated on ELISA, WB.
The antibody against ygcB was raised in rabbit using the Recombinant Escherichia coli (strain K12) CAS3 protein as the immunogen. This antibody exists as a non-conjugated isotype IgG, Protein A/G purified. This antibody has been validated on ELISA, WB.
$299.00
Western Blot | Positive WB detected in Recombinant protein | All lanes: ygcB antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 31.8 kDa | Observed band size: 32 kDa
| Cat.No | ADC-05270A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | ygcB |
| Target Synonyms | ygcB antibody; cas3 antibody; b2761 antibody; JW2731 antibody; CRISPR-associated endonuclease/helicase Cas3 antibody; EC 3.1.-.- antibody; EC 3.6.4.- antibody | Form | Liquid |
| Species Reactivity | Escherichia coli (strain K12) | Isotype | IgG |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | Protein A/G purified |
| Conjugate | Non-conjugated | Application | ELISA, WB |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Escherichia coli (strain K12) CAS3 protein | Target Species | Escherichia coli (strain K12) |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P38036 |
Uniprot Id
P38036
Target Species
E.coli
Target Name
YGCB
Target Function
CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity.; Acts as an endonuclease, a 3'-5'exonuclease, and an ATP-dependent dsDNA helicase. Anneals and unwinds R-loops (in which crRNA binds the target DNA, displacing the noncomplementary strand). Unwinding requires ATP, annealing does not. Required along with the Cascade complex for resistance to bacteriophage lambda infection as well as the ability to cure CRISPR-encoding high-copy number plasmid. A Cas3-CasA fusion protein purified with the Cascade complex nicks target plasmid in the presence but not absence of Mg(2+), and degrades plasmid fully in the presence of Mg(2+) and ATP, suggesting the helicase activity is required for complete degradation.
Target Protein Families
CRISPR-associated nuclease Cas3-HD family; CRISPR-associated helicase Cas3 family
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