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The antibody against EIF4B was raised in rabbit using the Peptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB, IHC.
The antibody against EIF4B was raised in rabbit using the Peptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB, IHC.
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Western blot analysis of extracts from Hela cells untreated or treated with EGF using eIF4B(phospho-Ser422) Antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcinomatissue using eIF4B(Phospho-Ser422) Antibody(left) or the same antibody preincubated with blocking peptide(right).2
| Cat.No | ADC-43580A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | EIF4B |
| Form | Liquid | Species Reactivity | Human, Mouse |
| Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+) | Application | ELISA, IHC, WB |
| Storage | Upon receipt |
| Immunogen Description | Peptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P23588 |
Uniprot Id
P23588
Target Species
Human
Target Name
EIF4B
Target Full Name
Eukaryotic translation initiation factor 4B
Target Function
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Target Synonyms
2310046H11Rik; AL024095; C85189; EIF 4B; eIF-4B; EIF4B; Eukaryotic initiation factor 4B; Eukaryotic translation initiation factor 4B; IF4B_HUMAN; PRO1843
Target Background
Enables RNA binding activity. Predicted to be involved in eukaryotic translation initiation factor 4F complex assembly and formation of translation preinitiation complex. Located in cytosol. Biomarker of autism spectrum disorder and major depressive disorder.
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