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The antibody against SHC1 was raised in rabbit using the Peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y(p)-V-N derived from Human Shc1. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB, IHC, IF.
The antibody against SHC1 was raised in rabbit using the Peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y(p)-V-N derived from Human Shc1. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB, IHC, IF.
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Western blot analysis of extracts from 293 cells untreated or treated with PMA using Shc1(Phospho-Tyr427) Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc1(Phospho-Tyr427) Antibody(left) or the same antibody preincubated with blocking peptide(right).2
Immunofluorescence staining of methanol-fixed Hela cells using Shc1(Phospho-Tyr427) Antibody.3
| Cat.No | ADC-43601A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | SHC1 |
| Form | Liquid | Species Reactivity | Human, Mouse, Rat |
| Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+) | Application | ELISA, IF, IHC, WB |
| Storage | Upon receipt |
| Immunogen Description | Peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y(p)-V-N derived from Human Shc1. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P29353 |
Uniprot Id
P29353
Target Species
Human
Target Name
SHC1
Target Full Name
SHC-transforming protein 1
Target Function
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span. Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
Target Subcellular Location
Cytoplasm.; [Isoform p46Shc]: Mitochondrion matrix.
Target Tissue Specificity
Widely expressed. Expressed in neural stem cells but absent in mature neurons.
Target Synonyms
FLJ26504; p66; p66SHC; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC 1; SHC A; SHC adaptor protein 1; Shc; SHC transforming protein 1; SHC transforming protein; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; SHC1; SHC1_HUMAN; SHCA; Src homology 2 domain-containing-transforming protein C1
Target Background
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
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