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The antibody against PLD1 was raised in rabbit using the Peptide sequence around phosphorylation site of threonine 147(R-H-T(p)-F-R) derived from Human PLD1. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB.
The antibody against PLD1 was raised in rabbit using the Peptide sequence around phosphorylation site of threonine 147(R-H-T(p)-F-R) derived from Human PLD1. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB.
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Western blot analysis of extracts from HepG2 cells using PLD1 (Phospho-Thr147) Antibody.The lane on the right is treated with the antigen-specific peptide.
| Cat.No | ADC-42669A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | PLD1 |
| Target Synonyms | Choline phosphatase 1 antibody; hPLD1 antibody; Phosphatidylcholine-hydrolyzing phospholipase D1 antibody; Phospholipase D1 antibody; PLD 1 antibody; PLD1 antibody; PLD1_HUMAN antibody | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) |
| Application | ELISA, WB | Storage | Upon receipt |
| Immunogen Description | Peptide sequence around phosphorylation site of threonine 147(R-H-T(p)-F-R) derived from Human PLD1. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q13393 |
Uniprot Id
Q13393
Target Species
Human
Target Name
PLD1
Target Full Name
Phospholipase D1
Target Function
Function as phospholipase selective for phosphatidylcholine. Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.
Target Involvement
Cardiac valvular defect, developmental (CVDD)
Target Subcellular Location
Cytoplasm, perinuclear region. Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Late endosome membrane; Lipid-anchor; Cytoplasmic side.
Target Protein Families
Phospholipase D family
Target Tissue Specificity
Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
Target Synonyms
Choline phosphatase 1; hPLD1; Phosphatidylcholine-hydrolyzing phospholipase D1; Phospholipase D1; PLD 1; PLD1; PLD1_HUMAN
Target Background
This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties.
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