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The antibody against ADD1 was raised in rabbit using the Peptide sequence around aa.724~728 (T-P-S-F-L) derived from Human ADD1. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. This antibody has been validated on ELISA, WB, IHC, IF.
The antibody against ADD1 was raised in rabbit using the Peptide sequence around aa.724~728 (T-P-S-F-L) derived from Human ADD1. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. This antibody has been validated on ELISA, WB, IHC, IF.
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Western blot analysis of extracts from 293 and Hela cells using ADD1(Ab-726) Antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1(Ab-726) Antibody(left) or the same antibody preincubated with blocking peptide(right).2
Immunofluorescence staining of methanol-fixed Hela cells using ADD1(Ab-726) Antibody.3
| Cat.No | ADC-43399A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | ADD1 |
| Form | Liquid | Species Reactivity | Human, Mouse, Rat |
| Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+) | Purification Method | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Application | ELISA, IF, IHC, WB | Storage | Upon receipt |
| Immunogen Description | Peptide sequence around aa.724~728 (T-P-S-F-L) derived from Human ADD1. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P35611 |
Uniprot Id
P35611
Target Species
Human
Target Name
ADD1
Target Full Name
Alpha-adducin
Target Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Target Subcellular Location
Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Target Protein Families
Aldolase class II family, Adducin subfamily
Target Tissue Specificity
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Target Synonyms
ADD A; ADD1; ADDA; ADDA_HUMAN; Adducin 1 (alpha); Alpha adducin; Alpha-adducin; Erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; MGC3339; MGC44427; OTTHUMP00000151163; OTTHUMP00000151164; OTTHUMP00000151165; OTTHUMP00000218664; OTTHUMP00000218665
Target Background
Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C.
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