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The antibody against API5 was raised in rabbit using the Synthesized peptide derived from the C-terminal region of Human API5. as the immunogen. This antibody exists as a non-conjugated isotype IgG. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on WB, IHC, ELISA.
The antibody against API5 was raised in rabbit using the Synthesized peptide derived from the C-terminal region of Human API5. as the immunogen. This antibody exists as a non-conjugated isotype IgG. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on WB, IHC, ELISA.
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Western Blot analysis of HuvEc cells using API5 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using API5 Polyclonal Antibody2
| Cat.No | ADC-39315A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | API5 |
| Form | Liquid | Species Reactivity | Human, Mouse |
| Isotype | IgG | Storage Buffer | 0.5% BSA and 0.02% sodium azide., Liquid in PBS containing 50% glycerol |
| Purification Method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | Conjugate | Non-conjugated |
| Application | ELISA, IHC, WB | Storage | Upon receipt |
| Immunogen Description | Synthesized peptide derived from the C-terminal region of Human API5. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q9BZZ5 |
Uniprot Id
Q9BZZ5
Target Species
Human
Target Name
API5
Target Full Name
Apoptosis inhibitor 5
Target Function
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Target Subcellular Location
Nucleus. Cytoplasm. Note=Mainly nuclear. Can also be cytoplasmic.; [Isoform 3]: Cytoplasm.
Target Protein Families
API5 family
Target Tissue Specificity
Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma
Target Synonyms
AAC 11; AAC-11; AAC11; Antiapoptosis clone 11 protein; API 5; API-5; api5; API5 like 1; API5_HUMAN; API5L1; Apoptosis inhibitor 5; Cell migration inducing gene 8 protein; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2 interacting factor 2; Fibroblast growth factor 2-interacting factor; FIF; MIG 8; MIG8; Migration inducing protein MIG8; Protein XAGL
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