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The antibody against CHAF1A was raised in rabbit using the Synthesized peptide derived from the Internal region of Human CAF-1 p150. as the immunogen. This antibody exists as a non-conjugated isotype IgG. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on WB, ELISA.
The antibody against CHAF1A was raised in rabbit using the Synthesized peptide derived from the Internal region of Human CAF-1 p150. as the immunogen. This antibody exists as a non-conjugated isotype IgG. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on WB, ELISA.
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Western Blot analysis of K562 cells using CAF-1 p150 Polyclonal Antibody
| Cat.No | ADC-36701A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | CHAF1A |
| Form | Liquid | Species Reactivity | Human, Mouse |
| Isotype | IgG | Storage Buffer | 0.5% BSA and 0.02% sodium azide., Liquid in PBS containing 50% glycerol |
| Purification Method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | Conjugate | Non-conjugated |
| Application | ELISA, WB | Storage | Upon receipt |
| Immunogen Description | Synthesized peptide derived from the Internal region of Human CAF-1 p150. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q13111 |
Uniprot Id
Q13111
Target Species
Human
Target Name
CHAF1A
Target Full Name
Chromatin assembly factor 1 subunit A
Target Function
Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.
Target Subcellular Location
Nucleus. Note=DNA replication foci.
Target Protein Families
CHAF1A family
Target Synonyms
CAF 1; CAF 1 subunit A; CAF; CAF I 150 kDa subunit; CAF I p150; CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150; CAF1; CAF1A_HUMAN; CAF1B; CAF1P150; CHAF1A; Chromatin assembly factor 1 subunit A; Chromatin Assembly Factor 1 Subunit A p150; Chromatin assembly factor I (150 kDa); Chromatin assembly factor I 150 kDa; Chromatin assembly factor I p150 subunit; DCAF1; hp150; MGC71229; P150
Target Background
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).
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