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The antibody against GPD2 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 25-325 of human GPD2 (NP_000399.3) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
The antibody against GPD2 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 25-325 of human GPD2 (NP_000399.3) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.
Western blot analysis of various lysates, using GPD2 Rabbit pAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
| Cat.No | ADA-00322A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | GPD2 |
| Target Synonyms | GDH2; GPDM; mGDH; mGPDH; GPD2 | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | HeLa, MCF7, U-87 MG | Application | ELISA, WB |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 25-325 of human GPD2 (NP_000399.3). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | P43304 | Immunogen Sequence |
Uniprot Id
P43304
Target Species
Human
Target Name
GPD2
Target Full Name
Glycerol-3-phosphate dehydrogenase, mitochondrial
Target Function
Calcium-responsive mitochondrial glycerol-3-phosphate dehydrogenase which seems to be a key component of the pancreatic beta-cell glucose-sensing device.
Target Subcellular Location
Mitochondrion.
Target Protein Families
FAD-dependent glycerol-3-phosphate dehydrogenase family
Target Research Area
Others
Target Synonyms
GDH2; Glycerol 3 phosphate dehydrogenase 2 (mitochondrial); Glycerol 3 phosphate dehydrogenase 2; Glycerol 3 phosphate dehydrogenase mitochondrial; Glycerol-3-phosphate dehydrogenase; GPD 2; GPD M; GPD-M; gpd2; GPDH M; GPDH-M; GPDM; GPDM_HUMAN; mGPDH; mitochondrial; Mitochondrial glycerophosphate dehydrogenase; mtGPD; OTTHUMP00000162788; OTTHUMP00000204452; OTTHUMP00000204453; OTTHUMP00000204458
Target Background
The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.
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