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The antibody against MDC1 was raised in rabbit using the Peptide sequence around phosphorylation site of Serine 513(E-R-S(p)-Q-A) derived from Human MDC1. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, IHC.
The antibody against MDC1 was raised in rabbit using the Peptide sequence around phosphorylation site of Serine 513(E-R-S(p)-Q-A) derived from Human MDC1. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, IHC.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using MDC1 (Phospho-Ser513) antibody (left)or the same antibody preincubated with blocking peptide (right).
| Cat.No | ADC-42680A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | MDC1 |
| Form | Liquid | Species Reactivity | Human |
| Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) | Application | ELISA, IHC |
| Storage | Upon receipt |
| Immunogen Description | Peptide sequence around phosphorylation site of Serine 513(E-R-S(p)-Q-A) derived from Human MDC1. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q14676 |
Uniprot Id
Q14676
Target Species
Human
Target Name
MDC1
Target Full Name
Mediator of DNA damage checkpoint protein 1
Target Function
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
Target Subcellular Location
Nucleus. Chromosome. Note=Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AX. Colocalizes with APTX at sites of DNA double-strand breaks.
Target Tissue Specificity
Highly expressed in testis.
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
Homologue to Drosophila photoreceptor protein calphotin; MDC 1; Mdc1; MDC1_HUMAN; Mediation of DNA damage checkpoint 1; Mediator of DNA damage checkpoint 1; Mediator of DNA damage checkpoint protein 1; NFBD 1; NFBD1; Nuclear factor with BRCT domains 1; Nuclear Factor with BRCT Domains Protein 1
Target Background
The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
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