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The antibody against PHOSPHO1 was raised in rabbit using the Recombinant Human Phosphoethanolamine/phosphocholine phosphatase protein (1-267AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, IHC.
The antibody against PHOSPHO1 was raised in rabbit using the Recombinant Human Phosphoethanolamine/phosphocholine phosphatase protein (1-267AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, IHC.
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Immunohistochemistry of paraffin-embedded human heart tissue using PHOSPHO1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using PHOSPHO1 Antibody at dilution of 1:1002
| Cat.No | ADC-10286A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | PHOSPHO1 |
| Target Synonyms | PHOP1_HUMAN antibody; Phosphatase orphan 1 antibody; Phospho 1 antibody; phospho1 antibody; Phosphoethanolamine/phosphocholine phosphatase antibody | Form | Liquid |
| Species Reactivity | Human | Isotype | IgG |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | >95%, Protein G purified |
| Conjugate | Non-conjugated | Application | ELISA, IHC |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Human Phosphoethanolamine/phosphocholine phosphatase protein (1-267AA) | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q8TCT1 |
Uniprot Id
Q8TCT1
Target Species
Human
Target Name
PHOSPHO1
Target Full Name
Phosphoethanolamine/phosphocholine phosphatase
Target Function
Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization. Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix.
Target Subcellular Location
Extracellular vesicle.
Target Protein Families
HAD-like hydrolase superfamily, PHOSPHO family
Target Tissue Specificity
Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.
Target Research Area
Signal Transduction
Target Synonyms
PHOP1_HUMAN; Phosphatase orphan 1; Phospho 1 ; phospho1; Phosphoethanolamine/phosphocholine phosphatase
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