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The antibody against RASSF2 was raised in rabbit using the Recombinant Human Ras association domain-containing protein 2 protein (69-326AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IP.
The antibody against RASSF2 was raised in rabbit using the Recombinant Human Ras association domain-containing protein 2 protein (69-326AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IP.
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Western Blot | Positive WB detected in: Raji whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate | All lanes: RASSF2 antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 38, 18 kDa | Observed band size: 38 kDa
Immunoprecipitating RASSF2 in Raji whole cell lysate | Lane 1: Rabbit control IgG instead of RASSF2 Antibody in Raji whole cell lysate. | For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000) | Lane 2: RASSF2 Antibody (5ug) + Raji whole cell lysate (0.5mg) | Lane 3: Raji whole cell lysate (20ug)2
| Cat.No | ADC-01620A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | RASSF2 |
| Form | Liquid | Species Reactivity | Human |
| Isotype | IgG | Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 |
| Purification Method | >95%, Protein G purified | Conjugate | Non-conjugated |
| Application | ELISA, WB, IP | Storage | Upon receipt |
| Immunogen Description | Recombinant Human Ras association domain-containing protein 2 protein (69-326AA) | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P50749 |
Uniprot Id
P50749
Target Species
Human
Target Name
RASSF2
Target Full Name
Ras association domain-containing protein 2
Target Function
Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.
Target Subcellular Location
Nucleus. Cytoplasm. Chromosome, centromere, kinetochore. Note=Translocates to the cytoplasm in the presence of STK3/MST2 AND STK4/MST1.
Target Tissue Specificity
Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.
Target Synonyms
CENP 34; Centromere protein 34; Ras association (RalGDS/AF 6) domain family 2; Ras association (RalGDS/AF-6) domain family member 2; Ras association domain containing protein 2; Ras association domain-containing protein 2; RASF2_HUMAN; RASFADIN; RASSF2; RP4-599I11.1
Target Background
This gene encodes a protein that contains a Ras association domain. Similar to its cattle and sheep counterparts, this gene is located near the prion gene. Two alternatively spliced transcripts encoding the same isoform have been reported.
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