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The antibody against RGS16 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-202 of human RGS16 (NP_002919.3) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
The antibody against RGS16 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-202 of human RGS16 (NP_002919.3) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
Western blot analysis of Rat thymus, using RGS16 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
| Cat.No | ADA-10717A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | RGS16 |
| Target Synonyms | RGS-R; A28-RGS14; A28-RGS14P; RGS16 | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Rat thymus | Application | ELISA, WB, IHC-P |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-202 of human RGS16 (NP_002919.3). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | O15492 | Immunogen Sequence |
Uniprot Id
O15492
Target Species
Human
Target Name
RGS16
Target Full Name
Regulator of G-protein signaling 16
Target Function
Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. Plays an important role in the phototransduction cascade by regulating the lifetime and effective concentration of activated transducin alpha. May regulate extra and intracellular mitogenic signals.
Target Subcellular Location
Membrane; Lipid-anchor.
Target Tissue Specificity
Abundantly expressed in retina with lower levels of expression in most other tissues.
Target Research Area
Signal Transduction
Target Synonyms
A28 RGS14; A28 RGS14P; A28-RGS14P; HGNC:9997; hRGS-r; OTTHUMP00000033147; Regulator of G protein signaling 16; Regulator of G protein signalling 16; Regulator of G-protein signaling 16; Retinal-specific RGS; Retinally abundant regulator of G protein signaling; Retinally abundant regulator of G-protein signaling; RGS 16; RGS R; RGS-R; Rgs14; RGS16; RGS16_HUMAN; RGSR
Target Background
The protein encoded by this gene belongs to the 'regulator of G protein signaling' family. It inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade.
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