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The antibody against TSPAN15 was raised in rabbit using the Recombinant Human Tetraspanin-15 protein (115-235AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IF.
The antibody against TSPAN15 was raised in rabbit using the Recombinant Human Tetraspanin-15 protein (115-235AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IF.
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Immunofluorescence staining of A549 cell with TSPAN15 Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot Positive WB detected in: MCF7 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, JK whole cell lysate, 293 whole cell lysate All lanes: TSPAN15 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa2
| Cat.No | ADC-23130A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | TSPAN15 |
| Target Synonyms | TSPAN15; NET7; TM4SF15; UNQ677/PRO1311; Tetraspanin-15; Tspan-15; Tetraspan NET-7; Transmembrane 4 superfamily member 15 | Form | Liquid |
| Species Reactivity | Human | Isotype | IgG |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | Antigen affinity purified |
| Conjugate | Non-conjugated | Application | ELISA, IF, WB |
| Storage | Upon receipt |
| Immunogen Description | Recombinant Human Tetraspanin-15 protein (115-235AA) | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | O95858 |
Uniprot Id
O95858
Target Species
Human
Target Name
TSPAN15
Target Full Name
Tetraspanin-15
Target Function
Regulates maturation and trafficking of the transmembrane metalloprotease ADAM10. Promotes ADAM10-mediated cleavage of CDH2. Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity.
Target Subcellular Location
Cell membrane; Multi-pass membrane protein. Late endosome membrane.
Target Protein Families
Tetraspanin (TM4SF) family
Target Synonyms
TSPAN15; NET7; TM4SF15; UNQ677/PRO1311; Tetraspanin-15; Tspan-15; Tetraspan NET-7; Transmembrane 4 superfamily member 15
Target Background
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The use of alternate polyadenylation sites has been found for this gene.
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