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The antibody against VRK3 was raised in rabbit using the Synthesized peptide derived from the Internal region of Human VRK3. as the immunogen. This antibody exists as a non-conjugated isotype IgG. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on WB, ELISA.
The antibody against VRK3 was raised in rabbit using the Synthesized peptide derived from the Internal region of Human VRK3. as the immunogen. This antibody exists as a non-conjugated isotype IgG. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on WB, ELISA.
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Western Blot analysis of COLO cells using VRK3 Polyclonal Antibody
| Cat.No | ADC-34776A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | VRK3 |
| Form | Liquid | Species Reactivity | Human |
| Isotype | IgG | Storage Buffer | 0.5% BSA and 0.02% sodium azide., Liquid in PBS containing 50% glycerol |
| Purification Method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | Conjugate | Non-conjugated |
| Application | ELISA, WB | Storage | Upon receipt |
| Immunogen Description | Synthesized peptide derived from the Internal region of Human VRK3. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q8IV63 |
Uniprot Id
Q8IV63
Target Species
Human
Target Name
VRK3
Target Full Name
Serine/threonine-protein kinase VRK3
Target Function
Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus.
Target Subcellular Location
Nucleus.
Target Protein Families
Protein kinase superfamily, CK1 Ser/Thr protein kinase family, VRK subfamily
Target Synonyms
Inactive serine/threonine-protein kinase VRK3; Serine/threonine protein kinase VRK3; Serine/threonine-protein pseudokinase VRK3; Vaccinia related kinase 3; Vaccinia-related kinase 3; Vrk3; VRK3_HUMAN
Target Background
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. This gene is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms.
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