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The antibody against Pim1 was raised in rabbit using the Peptide sequence around phosphorylation site of tyrosine 309(A-A-V(p)-W-S) derived from Human Pim-1. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB.
The antibody against Pim1 was raised in rabbit using the Peptide sequence around phosphorylation site of tyrosine 309(A-A-V(p)-W-S) derived from Human Pim-1. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB.
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Western blot analysis of extracts from HUVEC cells treated with PMA using Pim-1 (Phospho-Tyr309) Antibody.The lane on the right is treated with the antigen-specific peptide.
| Cat.No | ADC-42781A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | PIM1 |
| Target Synonyms | Pim1 antibody; Pim-1 antibody; Serine/threonine-protein kinase pim-1 antibody; EC 2.7.11.1 antibody | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) |
| Application | ELISA, WB | Storage | Upon receipt |
| Immunogen Description | Peptide sequence around phosphorylation site of tyrosine 309(A-A-V(p)-W-S) derived from Human Pim-1. | Target Species | Mus musculus (Mouse) |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P06803 |
Uniprot Id
P06803
Target Species
Mouse
Target Name
PIM1
Target Full Name
Serine/threonine-protein kinase pim-1
Target Function
Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, another proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promotes cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B, induces 14-3-3 binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis. Also phosphorylates and activates the ATP-binding cassette transporter ABCG2, allowing resistance to drugs through their excretion from cells. Promotes brown adipocyte differentiation.
Target Involvement
Frequently activated by provirus insertion in murine leukemia virus-induced T-cell lymphomas.
Target Subcellular Location
Cytoplasm. Nucleus. Cell membrane. Note=Mainly located in the cytoplasm.
Target Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, PIM subfamily
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