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The antibody against Stat5a/Stat5b was raised in rabbit using the Peptide sequence around phosphorylation site of serine725/730(A-P-S(p)-P-V) derived from Human STAT5A/B. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB.
The antibody against Stat5a/Stat5b was raised in rabbit using the Peptide sequence around phosphorylation site of serine725/730(A-P-S(p)-P-V) derived from Human STAT5A/B. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB.
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Western blot analysis of extracts from Jurkat cells treated with EGF using Phospho-STAT5A/B (Ser725/730) antibody.The lane on the right is treated with the antigen-specific peptide.
| Cat.No | ADC-42824A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | STAT5A/STAT5B |
| Target Synonyms | Stat5a antibody; Mgf antibody; Mpf antibody; Signal transducer and activator of transcription 5A antibody; Mammary gland factor antibody | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) |
| Application | ELISA, WB | Storage | Upon receipt |
| Immunogen Description | Peptide sequence around phosphorylation site of serine725/730(A-P-S(p)-P-V) derived from Human STAT5A/B. | Target Species | Mus musculus (Mouse) |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P42230 |
Uniprot Id
P42230
Target Species
Mouse
Target Name
STAT5A
Target Full Name
Signal transducer and activator of transcription 5A
Target Function
Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Target Subcellular Location
Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation.
Target Protein Families
Transcription factor STAT family
Target Tissue Specificity
In the virgin, found in most tissues except brain and muscle. During lactation, abundantly found in mammary tissue, as well as in other secretory organs such as salivary gland and seminal vesicle.
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