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The antibody against GCLC was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human GCLC (NP_001489.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
The antibody against GCLC was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human GCLC (NP_001489.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
Western blot analysis of various lysates, using GCLC Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
| Cat.No | ADA-12825A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | GCLC |
| Target Synonyms | GCL; GCS; GLCL; GLCLC; GCLC | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.01% thimerosal, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | HeLa, Mouse liver | Application | ELISA, WB, IF/ICC |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human GCLC (NP_001489.1). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | P48506 | Immunogen Sequence |
Uniprot Id
P48506
Target Species
Human
Target Name
GCLC
Target Full Name
Glutamate--cysteine ligase catalytic subunit
Target Involvement
Hemolytic anemia due to gamma-glutamylcysteine synthetase deficiency (HAGGSD)
Target Protein Families
Glutamate--cysteine ligase type 3 family
Target Synonyms
EC 6.3.2.2; Gamma ECS; Gamma glutamylcysteine synthetase; Gamma-ECS; Gamma-glutamylcysteine synthetase; GCL; Gclc; GCS; GCS heavy chain; GLCL; GLCLC; Glutamate cysteine ligase catalytic subunit; Glutamate--cysteine ligase catalytic subunit; GSH1_HUMAN
Target Background
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
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