Chinese (Simplified)
English
German
Korean
Spanish
Sign up for an account to enjoy easy online shopping and instant order tracking.
The antibody against APAF1 was raised in rabbit using the Synthesized peptide derived from Human APAF-1-ALT. as the immunogen. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on ELISA, WB, IHC, IF.
The antibody against APAF1 was raised in rabbit using the Synthesized peptide derived from Human APAF-1-ALT. as the immunogen. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on ELISA, WB, IHC, IF.
$297.00
Western blot analysis of extracts from COLO205, using APAF-1-ALT antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using APAF-1-ALT antibody.2
| Cat.No | ADC-42461A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | APAF1 |
| Form | Liquid | Species Reactivity | Human |
| Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) | Purification Method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Application | ELISA, IF, IHC, WB | Storage | Upon receipt |
| Immunogen Description | Synthesized peptide derived from Human APAF-1-ALT. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | O14727-6 |
Uniprot Id
O14727
Target Species
Human
Target Name
APAF1
Target Full Name
Apoptotic protease-activating factor 1
Target Function
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Target Subcellular Location
Cytoplasm.
Target Tissue Specificity
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Target Research Area
Cancer
Target Synonyms
APAF 1; Apaf-1; APAF_HUMAN; Apaf1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor 1; Apoptotic protease activating factor; Apoptotic protease-activating factor 1; CED 4; CED4; KIAA0413
Target Background
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Free shipping on orders $999+
Advenit post multos Scudilo Scutariorum tribunus velament
We accept credit cards, PayPal, and bank wires
Responsive and knowledgable customer service via email, text or phone
Notification
