Rabbit anti-Mouse IFN-beta Polyclonal Antibody

The antibody against IFN-beta was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of mouse IFN-beta (NP_034640.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.

ADA-08140A

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Western blot analysis of recombinant Mouse IFN-beta Protein using IFN-beta Rabbit pAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 10ng/5ng/1ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

Specifications

Cat.No	ADA-08140A	Clonality	Polyclonal
Host Species	Rabbit	Target Name	IFN-beta
Target Synonyms	Ifb; IFNB; If1da1; IFN-beta	Form	Liquid
Species Reactivity	Human, Mouse	Isotype	IgG
Storage Buffer	50% Glycerol, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	Recombinant Mouse IFN-beta Protein	Application	ELISA, WB, IF/ICC

Immunogen Information

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of mouse IFN-beta (NP_034640.1)	Target Species	Mouse
Immunogen Sequence	MNNRWILHAAFLLCFSTTALSINYKQLQLQERTNIRKCQELLEQLNGKINLTYRADFKIPMEMTEKMQKSYTAFAIQEMLQNVFLVFRNNFSSTGWNETIVVRLLDELHQQTVFLKTVLEEKQEERLTWEMSSTALHLKSYYWRVQRYLKLMKYNSYAWMVVRAEIFRNFLIIRRLTRNFQN	Uniprot ID	P01575

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