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The antibody against PLD2 was raised in rabbit using the Peptide sequence around phosphorylation site of tyrosine 169(E-N-Y(p)-L-N) derived from Human PLD2. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB, IHC.
The antibody against PLD2 was raised in rabbit using the Peptide sequence around phosphorylation site of tyrosine 169(E-N-Y(p)-L-N) derived from Human PLD2. as the immunogen. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi This antibody has been validated on ELISA, WB, IHC.
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Western blot analysis of extracts from Jurkat cells treated with TNF using PLD2 (Phospho-Tyr169) Antibody.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody (left)or the same antibody preincubated with blocking peptide (right).2
| Cat.No | ADC-42674A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | PLD2 |
| Form | Liquid | Species Reactivity | Human, Mouse, Rat |
| Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) | Application | ELISA, IHC, WB |
| Storage | Upon receipt |
| Immunogen Description | Peptide sequence around phosphorylation site of tyrosine 169(E-N-Y(p)-L-N) derived from Human PLD2. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | O14939 |
Uniprot Id
O14939
Target Species
Human
Target Name
PLD2
Target Full Name
Phospholipase D2
Target Function
Function as phospholipase selective for phosphatidylcholine. May have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Target Subcellular Location
Cell membrane; Lipid-anchor.
Target Protein Families
Phospholipase D family
Target Tissue Specificity
Ubiquitous.
Target Synonyms
Choline phosphatase 2; EC 3.1.4.4 ; hPLD 2; hPLD2; mPLD2; Phosphatidylcholine hydrolyzing phospholipase D2; Phosphatidylcholine-hydrolyzing phospholipase D2; Phospholipase D2; PhospholipaseD2; PLD 2; PLD1C; Pld2; PLD2_HUMAN; Pldc; rPLD2
Target Background
The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4, 5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.
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