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The antibody against RNF144A was raised in rabbit using the Synthesized peptide derived from the Internal region of Human UBCE7IP4. as the immunogen. This antibody exists as a non-conjugated isotype IgG. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on WB, ELISA.
The antibody against RNF144A was raised in rabbit using the Synthesized peptide derived from the Internal region of Human UBCE7IP4. as the immunogen. This antibody exists as a non-conjugated isotype IgG. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on WB, ELISA.
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Western Blot analysis of COLO205 cells using UBCE7IP4 Polyclonal Antibody
| Cat.No | ADC-37409A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | RNF144A |
| Target Synonyms | RNF144A; KIAA0161; RNF144; UBCE7IP4; E3 ubiquitin-protein ligase RNF144A; RING finger protein 144A; UbcM4-interacting protein 4; Ubiquitin-conjugating enzyme 7-interacting protein 4 | Form | Liquid |
| Species Reactivity | Human, Mouse | Isotype | IgG |
| Storage Buffer | 0.5% BSA and 0.02% sodium azide., Liquid in PBS containing 50% glycerol | Purification Method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugate | Non-conjugated | Application | ELISA, WB |
| Storage | Upon receipt |
| Immunogen Description | Synthesized peptide derived from the Internal region of Human UBCE7IP4. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P50876 |
Uniprot Id
P50876
Target Species
Human
Target Name
RNF144A
Target Full Name
E3 ubiquitin-protein ligase RNF144A
Target Function
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC.
Target Subcellular Location
Cell membrane; Single-pass membrane protein. Cytoplasmic vesicle membrane.
Target Protein Families
RBR family, RNF144 subfamily
Target Synonyms
RNF144A; KIAA0161; RNF144; UBCE7IP4; E3 ubiquitin-protein ligase RNF144A; RING finger protein 144A; UbcM4-interacting protein 4; Ubiquitin-conjugating enzyme 7-interacting protein 4
Target Background
This gene encodes a member of a family of RING finger domain-containing E3 ubiquitin ligases that also includes parkin and parc. The expression of this gene is induced by DNA damage. The encoded protein interacts with the cytoplasmic DNA-dependent protein kinase, catalytic subunit (DNA-PKcs) and promotes its degradation through ubiquitination. The orthologous mouse protein has been shown to interact with a ubiquitin-conjugating enzyme involved in embryonic development.
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