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The antibody against MRPL12 was raised in rabbit using the Synthesized peptide derived from internal of Human MRPL12. as the immunogen. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on ELISA, WB.
The antibody against MRPL12 was raised in rabbit using the Synthesized peptide derived from internal of Human MRPL12. as the immunogen. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on ELISA, WB.
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Western blot analysis of extracts from COLO cells, using MRPL12 antibody.
| Cat.No | ADC-41578A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | MRPL12 |
| Target Synonyms | 39S ribosomal protein L12 antibody; 39S ribosomal protein L12 | Form | Liquid |
| Species Reactivity | Human | Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) |
| Purification Method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | Application | ELISA, WB |
| Storage | Upon receipt |
| Immunogen Description | Synthesized peptide derived from internal of Human MRPL12. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P52815 |
Uniprot Id
P52815
Target Species
Human
Target Name
MRPL12
Target Full Name
Large ribosomal subunit protein bL12m
Target Function
As a component of the mitochondrial large ribosomal subunit, it plays a role in mitochondrial translation. Associates with mitochondrial RNA polymerase to activate transcription.
Target Subcellular Location
Mitochondrion.
Target Protein Families
Bacterial ribosomal protein bL12 family
Target Research Area
Tags & Cell Markers
Target Synonyms
39S ribosomal protein L12; 39S ribosomal protein L12, mitochondrial; 5c5-2; FLJ60124; L12mt; MGC8610; mitochondrial; Mitochondrial ribosomal protein L12; MRP-L12; MRP-L31/34; MRPL12; MRPL7; MRPL7/L12; RM12_HUMAN; RPML12
Target Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.
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