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The antibody against SMYD2 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SMYD2 (NP_064582.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
The antibody against SMYD2 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SMYD2 (NP_064582.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
Western blot analysis of Hep G2, using SMYD2 Rabbit pAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.
| Cat.No | ADA-11101A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | SMYD2 |
| Target Synonyms | KMT3C; HSKM-B; ZMYND14; SMYD2 | Form | Liquid |
| Species Reactivity | Human, Mouse | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Hep G2 | Application | ELISA, WB, IF/ICC |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SMYD2 (NP_064582.2). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q9NRG4 | Immunogen Sequence |
Uniprot Id
Q9NRG4
Target Species
Human
Target Name
SMYD2
Target Full Name
N-lysine methyltransferase SMYD2
Target Function
Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 'Lys-4' (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.
Target Subcellular Location
Cytoplasm, cytosol. Nucleus.
Target Protein Families
Class V-like SAM-binding methyltransferase superfamily
Target Synonyms
Histone methyltransferase SMYD2; HSKM B; HSKM-B; HSKMB; KMT3C; Lysine N-methyltransferase 3C; MGC119305; N lysine methyltransferase SMYD2; N-lysine methyltransferase SMYD2; SET and MYND domain containing 2; SET and MYND domain containing protein 2; SET and MYND domain-containing protein 2; Smyd2; SMYD2_HUMAN; Zinc finger MYND domain containing 14; ZMYND14
Target Background
SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).
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