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The antibody against PRL was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 29-227 of human PRL (NP_001157030.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
The antibody against PRL was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 29-227 of human PRL (NP_001157030.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
Western blot analysis of extracts of normal 293F cells and 293F transfected with 293F-Prolactin-Avi-His-C protein, using PRL Rabbit pAb at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
| Cat.No | ADA-09994A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | Prl |
| Target Synonyms | GHA1; PRL | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | 293F-Prolactin-Avi-His-C | Application | ELISA, WB, IHC-P |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 29-227 of human PRL (NP_001157030.1). | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | LPICPGGAARCQVTLRDLFDRAVVLSHYIHNLSSEMFSEFDKRYTHGRGFITKAINSCHTSSLATPEDKEQAQQMNQKDFLSLIVSILRSWNEPLYHLVTEVRGMQEAPEAILSKAVEIEEQTKRLLEGMELIVSQVHPETKENEIYPVWSGLPSLQMADEESRLSAYYNLLHCLRRDSHKIDNYLKLLKCRIIHNNNC | Uniprot ID | P01236 |
Uniprot Id
P01236
Target Species
Human
Target Name
PRL
Target Full Name
Prolactin
Target Function
Prolactin acts primarily on the mammary gland by promoting lactation.
Target Subcellular Location
Secreted.
Target Protein Families
Somatotropin/prolactin family
Target Synonyms
GHA1; PRL
Target Background
This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein.
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