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The antibody against SARDH was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 300-560 of human SARDH (NP_009032.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
The antibody against SARDH was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 300-560 of human SARDH (NP_009032.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
Western blot analysis of various lysates, using SARDH Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 40s.
| Cat.No | ADA-00965A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | SARDH |
| Target Synonyms | SAR; SDH; SARD; BPR-2; DMGDHL1; SARDH | Form | Liquid |
| Species Reactivity | Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.01% thimerosal, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Mouse kidney, Mouse liver, Rat kidney, Rat liver | Application | ELISA, WB, IF/ICC |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 300-560 of human SARDH (NP_009032.2). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q9UL12 | Immunogen Sequence |
Uniprot Id
Q9UL12
Target Species
Human
Target Name
SARDH
Target Full Name
Sarcosine dehydrogenase, mitochondrial
Target Involvement
Sarcosinemia (SARCOS)
Target Subcellular Location
Mitochondrion matrix.
Target Protein Families
GcvT family
Target Synonyms
SARDH; DMGDHL1; Sarcosine dehydrogenase; mitochondrial; SarDH; EC 1.5.8.3; BPR-2
Target Background
This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described.
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