Rabbit anti-Human AP2B1 Monoclonal Antibody

Uniprot Id

P63010

Target Species

Human

Target Name

AP2B1

Target Full Name

AP-2 complex subunit beta

Target Function

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.

Target Subcellular Location

Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.

Target Protein Families

Adaptor complexes large subunit family

Target Tissue Specificity

Expressed in the brain (at protein level).

Target Synonyms

Adapter related protein complex 2 beta 1 subunit; Adapter-related protein complex 2 beta subunit; Adaptin, beta 2 (beta); Adaptor protein complex AP-2 subunit beta; Adaptor related protein complex 2, beta 1 subunit; ADTB2; AP-2 complex subunit beta; AP105B; AP2 BETA; Ap2b1; AP2B1_HUMAN; Beta adaptin; Beta-2-adaptin; Beta-adaptin; Beta2 adaptin; CLAPB1; Clathrin assembly protein complex 2 beta large chain; Clathrin associated/assembly/adaptor protein, large, beta 1; DKFZp781K0743; Plasma membrane adaptor HA2/AP2 adaptin beta subunit

Target Background

The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.