Rabbit anti-Mouse CD25 Monoclonal Antibody

The antibody against CD25 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 22-236 of mouse CD25 (NP_032393.3) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, FC, ELISA.

ADA-15467A

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Western blot analysis of lysates from Mouse spleen, using CD25 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

Specifications

Cat.No	ADA-15467A	Clonality	Monoclonal
Host Species	Rabbit	Target Name	CD25
Target Synonyms	CD25; Il2r; Ly-43	Form	Liquid
Species Reactivity	Mouse	Isotype	IgG
Storage Buffer	50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3.	Purification Method	Affinity purification
Positive Samples	Mouse spleen	Application	ELISA, WB, FC

Immunogen Information

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 22-236 of mouse CD25 (NP_032393.3)	Target Species	Mouse
Uniprot ID	P01590	Immunogen Sequence

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