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The antibody against CGRP was raised in Rabbit using a synthetic peptide corresponding to a sequence within amino acids 25-125 of human CGRP (NP_001029125.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
The antibody against CGRP was raised in Rabbit using a synthetic peptide corresponding to a sequence within amino acids 25-125 of human CGRP (NP_001029125.1) as the immunogen. The monoclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IF/ICC, ELISA.
Western blot analysis of lysates from control 293T cells and CGRP-293T transfected cells, using CGRP Rabbit mAb at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
| Cat.No | ADA-15502A | Clonality | Monoclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | CGRP |
| Target Synonyms | CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I; CGRP-alpha | Form | Liquid |
| Species Reactivity | Human | Isotype | IgG |
| Storage Buffer | 50% Glycerol, 0.05% BSA, PBS with 0.05% proclin300, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | 293T transfected with CGRP | Application | ELISA, WB, IF/ICC |
| Immunogen Description | A synthetic peptide corresponding to a sequence within amino acids 25-125 of human CGRP (NP_001029125.1). | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | AAPFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSRIIAQKRACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKAFGRRRRD | Uniprot ID | P06881 |
Uniprot Id
P06881
Target Species
Human
Target Name
CALCA
Target Full Name
Calcitonin gene-related peptide 1
Target Function
CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.
Target Subcellular Location
Secreted.
Target Protein Families
Calcitonin family
Target Research Area
Signal Transduction
Target Synonyms
CALCA; CALC1Calcitonin gene-related peptide 1; Alpha-type CGRP; Calcitonin gene-related peptide I; CGRP-I
Target Background
This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
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