Recombinant Human?Decapping?and?exoribonuclease?protein (DXO)

Cat.No	ACP00713	Target Name	DXO
Target Synonyms	DXO;5'-3' exoribonuclease DXO;Dom-3 homolog Z;NAD-capped RNA hydrolase DXO;DeNADding enzyme DXO	Form	Liquid or Lyophilized powder
Expression System	E.coli	Expression Range	1-396aa
Mol Weight	51.8 kDa	Protein Length	Full length
Purity	Greater than 90% as determined by SDS-PAGE.	Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP00713

Target Name

DXO

Target Synonyms

DXO;5'-3' exoribonuclease DXO;Dom-3 homolog Z;NAD-capped RNA hydrolase DXO;DeNADding enzyme DXO

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

1-396aa

Mol Weight

51.8 kDa

Protein Length

Full length

Purity

Greater than 90% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	O77932

Background Information

Uniprot Id

O77932
Target Species

Human
Target Name

DXO
Target Full Name

Decapping and exoribonuclease protein
Target Function

Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA. The NAD-cap is present at the 5'-end of some RNAs and snoRNAs. In contrast to the canonical 5'-end N7 methylguanosine (m7G) cap, the NAD cap promotes mRNA decay. Preferentially acts on NAD-capped transcripts in response to environmental stress. Also acts as a non-canonical decapping enzyme that removes the entire cap structure of m7G capped or incompletely capped RNAs and mediates their subsequent degradation. Specifically degrades pre-mRNAs with a defective 5'-end m7G cap and is part of a pre-mRNA capping quality control. Has decapping activity toward incomplete 5'-end m7G cap mRNAs such as unmethylated 5'-end-capped RNA (cap0), while it has no activity toward 2'-O-ribose methylated m7G cap (cap1). In contrast to canonical decapping enzymes DCP2 and NUDT16, which cleave the cap within the triphosphate linkage, the decapping activity releases the entire cap structure GpppN and a 5'-end monophosphate RNA. Also has 5'-3' exoribonuclease activities: The 5'-end monophosphate RNA is then degraded by the 5'-3' exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Also possesses RNA 5'-pyrophosphohydrolase activity by hydrolyzing the 5'-end triphosphate to release pyrophosphates. Exhibits decapping activity towards FAD-capped RNAs. Exhibits decapping activity towards dpCoA-capped RNAs in vitro.
Target Subcellular Location

Nucleus.
Target Protein Families

DXO/Dom3Z family
Target Tissue Specificity

Ubiquitously expressed.
Target Research Area

Signal Transduction
Target Synonyms

Dom 3 homolog Z; DOM 3Z; Dom-3 homolog Z; DOM3 C. elegans homolog of Z; Dom3 homolog Z; DOM3L; Dom3z; DOM3Z_HUMAN; NG6; Protein Dom3Z; RAI1; RAT1-INTERACTING PROTEIN 1

Recombinant Human?Decapping?and?exoribonuclease?protein (DXO)

Specifications

Immunogen Information

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