Chinese (Simplified)
English
German
Korean
Spanish
Sign up for an account to enjoy easy online shopping and instant order tracking.
The recombinant Human CD3G protein production is achieved through the manipulation of desired gene expression in e.coli cells. The sequence (23-116aa) of foreign DNA is fused to an expression vector and then transformed into e.coli cells. The positive cells are selected and cultured to induce the expression of the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The recombinant Human CD3G protein is subjected to affinity purification. Its purity is over 90%, as measured by SDS-PAGE.
The recombinant Human CD3G protein production is achieved through the manipulation of desired gene expression in e.coli cells. The sequence (23-116aa) of foreign DNA is fused to an expression vector and then transformed into e.coli cells. The positive cells are selected and cultured to induce the expression of the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The recombinant Human CD3G protein is subjected to affinity purification. Its purity is over 90%, as measured by SDS-PAGE.
| Cat.No | ACP01154 | Target Name | CD3G |
|---|---|---|---|
| Target Synonyms | T-cell receptor T3 gamma chain;CD antigen CD3g | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 23-116aa |
| Mol Weight | 18.2 kDa | Protein Length | Partial |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P09693 |
|---|
Uniprot Id
P09693
Target Species
Human
Target Name
CD3G
Target Full Name
T-cell surface glycoprotein CD3 gamma chain
Target Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.
Target Involvement
Immunodeficiency 17 (IMD17)
Target Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Target Research Area
Immunology
Target Synonyms
CD3G; T3G; T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; CD antigen CD3g
Target Background
The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.
Free shipping on orders $999+
Advenit post multos Scudilo Scutariorum tribunus velament
We accept credit cards, PayPal, and bank wires
Responsive and knowledgable customer service via email, text or phone
Notification
