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The expression of recombinant Human SEPHS2 protein involves transfecting e.coli cells with a DNA expression vector carrying the gene for the protein of interest (2-448aa(U60S)). After transfection, the cells are cultured to produce the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The recombinant Human SEPHS2 protein is collected and purified from the cell lysate through affinity purification, attaining a purity greater than 90%, as determined by SDS-PAGE.
The expression of recombinant Human SEPHS2 protein involves transfecting e.coli cells with a DNA expression vector carrying the gene for the protein of interest (2-448aa(U60S)). After transfection, the cells are cultured to produce the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The recombinant Human SEPHS2 protein is collected and purified from the cell lysate through affinity purification, attaining a purity greater than 90%, as determined by SDS-PAGE.
| Cat.No | ACP01230 | Target Name | SEPHS2 |
|---|---|---|---|
| Target Synonyms | (Selenium donor protein 2)(Selenophosphate synthase 2) | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 2-448aa(U60S) |
| Mol Weight | 54.6 kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q99611 |
|---|
Uniprot Id
Q99611
Target Species
Human
Target Name
SEPHS2
Target Full Name
Selenide, water dikinase 2
Target Function
Synthesizes selenophosphate from selenide and ATP.
Target Protein Families
Selenophosphate synthase 1 family, Class I subfamily
Target Research Area
Others
Target Synonyms
SEPHS2; SPS2; Selenide; water dikinase 2; EC 2.7.9.3; Selenium donor protein 2; Selenophosphate synthase 2
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