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In the process of expressing the recombinant Human NTF3 protein, mammalian cells are transfected with a DNA expression vector that carries the gene encoding the desired protein (139-257aa). The cells are then cultured to induce the expression of the intended protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant Human NTF3 protein is collected and purified from the cell lysate through affinity purification, with its purity surpassing 85%, as assessed by SDS-PAGE.
In the process of expressing the recombinant Human NTF3 protein, mammalian cells are transfected with a DNA expression vector that carries the gene encoding the desired protein (139-257aa). The cells are then cultured to induce the expression of the intended protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant Human NTF3 protein is collected and purified from the cell lysate through affinity purification, with its purity surpassing 85%, as assessed by SDS-PAGE.
| Cat.No | ACP01761 | Target Name | NTF3 |
|---|---|---|---|
| Target Synonyms | (NT-3)(HDNF)(Nerve growth factor 2)(NGF-2)(Neurotrophic factor) | Form | Liquid or Lyophilized powder |
| Expression System | Mammalian cell | Expression Range | 139-257aa |
| Mol Weight | 18.7 kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P20783 |
|---|
Uniprot Id
P20783
Target Species
Human
Target Name
NTF3
Target Full Name
Neurotrophin-3
Target Function
Seems to promote the survival of visceral and proprioceptive sensory neurons.
Target Subcellular Location
Secreted.
Target Protein Families
NGF-beta family
Target Tissue Specificity
Brain and peripheral tissues.
Target Research Area
Neuroscience
Target Synonyms
HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; Neurotrophin 3 precursor; Neurotrophin-3; Neurotrophin3; NGF 2; NGF-2; NGF2; NT 3; NT-3; NT3; NTF 3; NTF3; NTF3_HUMAN
Target Background
The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
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