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The production of the recombinant human mitochondrial thiamine pyrophosphate carrier (SLC25A19) is carried out in an in vitro E. coli expression system. The gene fragment encoding the full-length human SLC25A19 protein (1-320aa), along with an N-terminal 10xHis-tag for purification purposes, is incorporated into an expression vector. In the cell-free system, the vector construct is mixed with an E. coli cell lysate that contains the necessary cellular components for transcription and translation. The mixture is then incubated under appropriate conditions to allow for the expression of the recombinant SLC25A19 protein. Following the expression, the recombinant SLC25A19 protein is obtained from the cell-free expression system. Purity analysis using SDS-PAGE demonstrates that the recombinant human SLC25A19 protein exhibits a purity level of up to 85%.
The production of the recombinant human mitochondrial thiamine pyrophosphate carrier (SLC25A19) is carried out in an in vitro E. coli expression system. The gene fragment encoding the full-length human SLC25A19 protein (1-320aa), along with an N-terminal 10xHis-tag for purification purposes, is incorporated into an expression vector. In the cell-free system, the vector construct is mixed with an E. coli cell lysate that contains the necessary cellular components for transcription and translation. The mixture is then incubated under appropriate conditions to allow for the expression of the recombinant SLC25A19 protein. Following the expression, the recombinant SLC25A19 protein is obtained from the cell-free expression system. Purity analysis using SDS-PAGE demonstrates that the recombinant human SLC25A19 protein exhibits a purity level of up to 85%.
| Cat.No | ACP02185 | Target Name | SLC25A19 |
|---|---|---|---|
| Target Synonyms | (Mitochondrial uncoupling protein 1)(Solute carrier family 25 member 19) | Form | Liquid or Lyophilized powder |
| Expression System | in vitro E.coli expression system | Expression Range | 1-320aa |
| Mol Weight | 37.0 kDa | Protein Length | Full length |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9HC21 |
|---|
Uniprot Id
Q9HC21
Target Species
Human
Target Name
SLC25A19
Target Full Name
Mitochondrial thiamine pyrophosphate carrier
Target Function
Mitochondrial transporter mediating uptake of thiamine pyrophosphate (ThPP) into mitochondria.
Target Involvement
Microcephaly, Amish type (MCPHA); Thiamine metabolism dysfunction syndrome 4, bilateral striatal degeneration and progressive polyneuropathy type (THMD4)
Target Subcellular Location
Mitochondrion inner membrane; Multi-pass membrane protein.
Target Protein Families
Mitochondrial carrier (TC 2.A.29) family
Target Tissue Specificity
Expressed in all tissues examined except for placenta. Highest levels in colon, kidney, lung, testis, spleen, and brain.
Target Research Area
Others
Target Synonyms
SLC25A19; DNC; MUP1; Mitochondrial thiamine pyrophosphate carrier; Mitochondrial uncoupling protein 1; Solute carrier family 25 member 19
Target Background
This gene encodes a mitochondrial protein that is a member of the solute carrier family. Although this protein was initially thought to be the mitochondrial deoxynucleotide carrier involved in the uptake of deoxynucleotides into the matrix of the mitochondria, further studies have demonstrated that this protein instead functions as the mitochondrial thiamine pyrophosphate carrier, which transports thiamine pyrophosphates into mitochondria. Mutations in this gene cause microcephaly, Amish type, a metabolic disease that results in severe congenital microcephaly, severe 2-ketoglutaric aciduria, and death within the first year. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene.
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