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Recombinant Human DDB1- and CUL4-associated factor 16(DCAF16) is a full length protein expressed with N-terminal 10xHis-tagged and C-terminal Myc-tagged in the E.coli. Its expression region corresponds to 1-216aa of human DCAF16 protein. Its purity was determined by SDS-PAGE and reached up to 85%. Under reducing conditions, a molecular weight band of around 35 kDa was visualized on the gel. Furthermore, this protein underwent validation via the LC-MS/MS analysis. This recombinant DCAF16 protein may be used to synthesize antibodies against DCAF16 or on the studies of DCAF16-associated signal transduction. DCAF16 is a gene on chromosome 4p15.31 that encodes a protein thought to function as a substrate recognition component for CUL4-DDB1 E3 ubiquitin-protein ligase complex, which mediates ubiquitination and proteasome-dependent degradation of nuclear proteins. This protein is involved in the pathway protein ubiquitination, which is part of protein modification.
Recombinant Human DDB1- and CUL4-associated factor 16(DCAF16) is a full length protein expressed with N-terminal 10xHis-tagged and C-terminal Myc-tagged in the E.coli. Its expression region corresponds to 1-216aa of human DCAF16 protein. Its purity was determined by SDS-PAGE and reached up to 85%. Under reducing conditions, a molecular weight band of around 35 kDa was visualized on the gel. Furthermore, this protein underwent validation via the LC-MS/MS analysis. This recombinant DCAF16 protein may be used to synthesize antibodies against DCAF16 or on the studies of DCAF16-associated signal transduction.
DCAF16 is a gene on chromosome 4p15.31 that encodes a protein thought to function as a substrate recognition component for CUL4-DDB1 E3 ubiquitin-protein ligase complex, which mediates ubiquitination and proteasome-dependent degradation of nuclear proteins. This protein is involved in the pathway protein ubiquitination, which is part of protein modification.
| Cat.No | ACP03208 | Target Name | DCAF16 |
|---|---|---|---|
| Target Synonyms | DCAF16; C4orf30DDB1- and CUL4-associated factor 16 | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-216aa |
| Mol Weight | 31.2 kDa | Protein Length | Full length |
| Purity | Greater than 85% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q9NXF7 |
|---|
Uniprot Id
Q9NXF7
Target Species
Human
Target Name
DCAF16
Target Full Name
DDB1- and CUL4-associated factor 16
Target Function
Functions as a substrate recognition component for CUL4-DDB1 E3 ubiquitin-protein ligase complex, which mediates ubiquitination and proteasome-dependent degradation of nuclear proteins.
Target Subcellular Location
Nucleus.
Target Research Area
Others
Target Synonyms
DCAF16; C4orf30DDB1- and CUL4-associated factor 16
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