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Amino acids 1-267 form the expressed segment for recombinant Human PHOSPHO1. The expected molecular weight for the PHOSPHO1 protein is calculated to be 33.7 kDa. Expression of this PHOSPHO1 protein is conducted in e.coli. Fusion of the N-terminal 6xHis tag into the PHOSPHO1 encoding gene fragment was conducted, allowing for easier detection and purification of the PHOSPHO1 protein in subsequent stages.The human phosphoethanolamine/phosphocholine phosphatase (PHOSPHO1) primarily functions in regulating skeletal mineralization by hydrolyzing phosphocholine and phosphoethanolamine. It is involved in the initiation of hydroxyapatite crystal formation during bone development. In bone and mineralization research, studying PHOSPHO1 contributes to understanding the molecular mechanisms involved in bone mineralization and may have implications for bone-related disorders. PHOSPHO1's involvement in mineralization processes makes it relevant in dental research as well. Investigating PHOSPHO1 provides insights into skeletal biology, mineral homeostasis, and potential applications in developing therapeutic strategies for skeletal disorders, enhancing the understanding of mineralization processes in different physiological and pathological contexts.
Amino acids 1-267 form the expressed segment for recombinant Human PHOSPHO1. The expected molecular weight for the PHOSPHO1 protein is calculated to be 33.7 kDa. Expression of this PHOSPHO1 protein is conducted in e.coli. Fusion of the N-terminal 6xHis tag into the PHOSPHO1 encoding gene fragment was conducted, allowing for easier detection and purification of the PHOSPHO1 protein in subsequent stages.The human phosphoethanolamine/phosphocholine phosphatase (PHOSPHO1) primarily functions in regulating skeletal mineralization by hydrolyzing phosphocholine and phosphoethanolamine. It is involved in the initiation of hydroxyapatite crystal formation during bone development. In bone and mineralization research, studying PHOSPHO1 contributes to understanding the molecular mechanisms involved in bone mineralization and may have implications for bone-related disorders. PHOSPHO1’s involvement in mineralization processes makes it relevant in dental research as well. Investigating PHOSPHO1 provides insights into skeletal biology, mineral homeostasis, and potential applications in developing therapeutic strategies for skeletal disorders, enhancing the understanding of mineralization processes in different physiological and pathological contexts.
| Cat.No | ACP03711 | Target Name | PHOSPHO1 |
|---|---|---|---|
| Target Synonyms | PHOP1_HUMAN; Phosphatase orphan 1; Phospho 1 ; phospho1; Phosphoethanolamine/phosphocholine phosphatase | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 1-267aa |
| Mol Weight | 33.7kDa | Protein Length | Full length |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | Q8TCT1 |
|---|
Uniprot Id
Q8TCT1
Target Species
Human
Target Name
PHOSPHO1
Target Full Name
Phosphoethanolamine/phosphocholine phosphatase
Target Function
Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization. Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix.
Target Subcellular Location
Extracellular vesicle.
Target Protein Families
HAD-like hydrolase superfamily, PHOSPHO family
Target Tissue Specificity
Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.
Target Research Area
Signal Transduction
Target Synonyms
PHOP1_HUMAN; Phosphatase orphan 1; Phospho 1 ; phospho1; Phosphoethanolamine/phosphocholine phosphatase
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