Recombinant Human Histone deacetylase 3 (HDAC3)

Amino acids 1-428 constitute the expression domain of recombinant Human HDAC3. This HDAC3 protein is expected to have a theoretical molecular weight of 64.8 kDa. Expression of this HDAC3 protein is conducted in e.coli. The HDAC3 gene fragment has been modified by fusing the N-terminal 6xHis-SUMO tag, providing convenience in detecting and purifying the recombinant HDAC3 protein during the following stages.Histone deacetylase 3 (HDAC3) is a crucial enzyme involved in epigenetic regulation by catalyzing the removal of acetyl groups from histone proteins. As part of the histone deacetylase family, HDAC3 plays a key role in controlling gene expression and chromatin structure. It is essential for various cellular processes, including cell cycle progression, differentiation, and apoptosis. HDAC3 is often found in multi-protein complexes and is implicated in the regulation of diverse biological pathways. Dysregulation of HDAC3 has been associated with several diseases, including cancer and neurological disorders. Targeting HDAC3 has become a focus in drug development for potential therapeutic interventions, making it a subject of interest in both basic research and clinical studies.

ACP04052

Number

Cat.No	ACP04052	Target Name	HDAC3
Target Synonyms	HD3; HDAC 3; HDAC3; HDAC3_HUMAN; Histone deacetylase 3; RPD3 2; RPD3; RPD3-2; SMAP45	Form	Liquid or Lyophilized powder
Expression System	E.coli	Expression Range	1-428aa
Mol Weight	64.8kDa	Protein Length	Full length
Purity	Greater than 90% as determined by SDS-PAGE.	Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP04052

Target Name

HDAC3

Target Synonyms

HD3; HDAC 3; HDAC3; HDAC3_HUMAN; Histone deacetylase 3; RPD3 2; RPD3; RPD3-2; SMAP45

Form

Liquid or Lyophilized powder

Expression System

E.coli

Expression Range

1-428aa

Mol Weight

64.8kDa

Protein Length

Full length

Purity

Greater than 90% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	O15379

Background Information

Uniprot Id

O15379
Target Species

Human
Target Name

HDAC3
Target Full Name

Histone deacetylase 3
Target Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress. Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner. During the activation phase, promotes the accumulation of ubiquitinated ARNTL/BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and ARNTL/BMAL1. The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene ARTNL/BMAL1 and the genes involved in lipid metabolism in the liver. Serves as a corepressor of RARA, causing its deacetylation and inhibition of RARE DNA element binding. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response. Interacts with SETD5.
Target Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, cytosol.
Target Protein Families

Histone deacetylase family, HD type 1 subfamily
Target Tissue Specificity

Widely expressed.
Target Research Area

Transcription
Target Synonyms

HD3; HDAC 3; HDAC3; HDAC3_HUMAN; Histone deacetylase 3; RPD3 2; RPD3; RPD3-2; SMAP45
Target Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Recombinant Human Histone deacetylase 3 (HDAC3)

Specifications

Immunogen Information

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