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Insertion of the gene encoding the Human AHCY protein (2-432aa) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into e.coli cells. Positive e.coli cells are selected relying on their ability to survive in the presence of a specific antibiotic. The e.coli cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal 6xHis-SUMO tag is attached to the protein. Following expression, the recombinant Human AHCY protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Human AHCY protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 90%.
Insertion of the gene encoding the Human AHCY protein (2-432aa) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into e.coli cells. Positive e.coli cells are selected relying on their ability to survive in the presence of a specific antibiotic. The e.coli cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal 6xHis-SUMO tag is attached to the protein. Following expression, the recombinant Human AHCY protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Human AHCY protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 90%.
| Cat.No | ACP04228 | Target Name | AHCY |
|---|---|---|---|
| Target Synonyms | Adenosylhomocysteinase; AdoHcyase; ahcY; S adenosyl L homocysteine hydrolase; S adenosylhomocysteine hydrolase; S-adenosyl-L-homocysteine hydrolase; SAHH; SAHH_HUMAN | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 2-432aa |
| Mol Weight | 63.6kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P23526 |
|---|
Uniprot Id
P23526
Target Species
Human
Target Name
AHCY
Target Full Name
Adenosylhomocysteinase
Target Function
Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
Target Involvement
Hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD)
Target Subcellular Location
Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target Protein Families
Adenosylhomocysteinase family
Target Research Area
Metabolism
Target Synonyms
Adenosylhomocysteinase; AdoHcyase; ahcY; S adenosyl L homocysteine hydrolase; S adenosylhomocysteine hydrolase; S-adenosyl-L-homocysteine hydrolase; SAHH; SAHH_HUMAN
Target Background
S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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