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Cloning the gene encoding the Human HIST1H2AG protein (2-130aa) into a plasmid vector leads to the formation of recombinant plasmid. The resulting recombination plasmid is transformed into e.coli cells. e.coli cells containing the recombinant plasmid survive in the presence of a specific antibiotic and are selected to be cultured under conditions conducive to the expression of the gene of interest. A N-terminal 6xHis tag is linked to the protein. Following expression, the recombinant Human HIST1H2AG protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human HIST1H2AG protein, demonstrating a purity exceeding 90%.
Cloning the gene encoding the Human HIST1H2AG protein (2-130aa) into a plasmid vector leads to the formation of recombinant plasmid. The resulting recombination plasmid is transformed into e.coli cells. e.coli cells containing the recombinant plasmid survive in the presence of a specific antibiotic and are selected to be cultured under conditions conducive to the expression of the gene of interest. A N-terminal 6xHis tag is linked to the protein. Following expression, the recombinant Human HIST1H2AG protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human HIST1H2AG protein, demonstrating a purity exceeding 90%.
| Cat.No | ACP04283 | Target Name | HIST1H2AG |
|---|---|---|---|
| Target Synonyms | H2AC11; H2AFP; HIST1H2AG;; H2AC13; H2AFC; HIST1H2AI;; H2AC15; H2AFD; HIST1H2AK;; H2AC16; H2AFI; HIST1H2AL;; H2AC17; H2AFN; HIST1H2AMHistone H2A type 1; H2A.1; Histone H2A/ptl | Form | Liquid or Lyophilized powder |
| Expression System | E.coli | Expression Range | 2-130aa |
| Mol Weight | 18.0kDa | Protein Length | Full Length of Mature Protein |
| Purity | Greater than 90% as determined by SDS-PAGE. | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P0C0S8 |
|---|
Uniprot Id
P0C0S8
Target Species
Human
Target Name
H2AC11
Target Full Name
Histone H2A type 1
Target Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Target Subcellular Location
Nucleus. Chromosome.
Target Protein Families
Histone H2A family
Target Research Area
Epigenetics and Nuclear Signaling
Target Synonyms
H2AC11; H2AFP; HIST1H2AG;; H2AC13; H2AFC; HIST1H2AI;; H2AC15; H2AFD; HIST1H2AK;; H2AC16; H2AFI; HIST1H2AL;; H2AC17; H2AFN; HIST1H2AMHistone H2A type 1; H2A.1; Histone H2A/ptl
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