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The recombinant Human PI3 is a yeast-expressed (61-117aa) protein with N-terminal 6xHis tag. The purity is 90%+ measured by SDS-PAGE. The highly developed genetic system, ease of use, reduced time input, and costs have made Pichia Pastoris an attractive organism for the expression and production of recombinant proteins. So we choose the Yeast?system to express this recombinant PI3 protein, which is able to carry specifically designed plasmids, and the plasmid used consists of restriction sites that can be used to insert the gene sequence of interest. Transformation of yeasts with the plasmid produces the desired protein and can be appropriately scaled up.PI3, also called Elafin, is an endogenous serine protease inhibitor that constitutes the epithelial barrier against neutrophil elastase (NE) and protease 3 (PR3). It can reduce airway mucus hypersecretion and decrease inflammatory lung injury. In addition to having protease inhibitory activity, Elafin also exerts several functions such as anti-inflammation, antimicrobe, antiproliferation, immune modulation, vascular remodeling, and wound healing. It is downregulated in several inflammatory diseases, including acute respiratory distress syndrome, inflammatory bowel disease, and acute lung injury. Elafin is differentially expressed in several tumors. Enhanced Elafin expression is associated with the differentiation of esophageal cancer. While downregulation of Elafin is detected in ductal carcinoma in situ, invasion breast cancer, and invasion ovarian cancer.
The recombinant Human PI3 is a yeast-expressed (61-117aa) protein with N-terminal 6xHis tag. The purity is 90%+ measured by SDS-PAGE. The highly developed genetic system, ease of use, reduced time input, and costs have made Pichia Pastoris an attractive organism for the expression and production of recombinant proteins. So we choose the Yeast?system to express this recombinant PI3 protein, which is able to carry specifically designed plasmids, and the plasmid used consists of restriction sites that can be used to insert the gene sequence of interest. Transformation of yeasts with the plasmid produces the desired protein and can be appropriately scaled up.PI3, also called Elafin, is an endogenous serine protease inhibitor that constitutes the epithelial barrier against neutrophil elastase (NE) and protease 3 (PR3). It can reduce airway mucus hypersecretion and decrease inflammatory lung injury. In addition to having protease inhibitory activity, Elafin also exerts several functions such as anti-inflammation, antimicrobe, antiproliferation, immune modulation, vascular remodeling, and wound healing. It is downregulated in several inflammatory diseases, including acute respiratory distress syndrome, inflammatory bowel disease, and acute lung injury. Elafin is differentially expressed in several tumors. Enhanced Elafin expression is associated with the differentiation of esophageal cancer. While downregulation of Elafin is detected in ductal carcinoma in situ, invasion breast cancer, and invasion ovarian cancer.
| Cat.No | ACP04823 | Target Name | PI3 |
|---|---|---|---|
| Form | Liquid or Lyophilized powder | Expression System | Yeast |
| Expression Range | 61-117aa | Mol Weight | 8.0kDa |
| Protein Length | Full Length of Mature Protein | Purity | Greater than 90% as determined by SDS-PAGE. |
| Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P19957 |
|---|
Uniprot Id
P19957
Target Species
Human
Target Name
PI3
Target Full Name
Elafin
Target Function
Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Has been shown to inhibit the alpha-4-beta-2/CHRNA2-CHRNB2 nicotinic acetylcholine receptor and to produce a weak inhibition on Kv11.1/KCNH2/ERG1 and on the transient receptor potential cation channel subfamily V member 1 (TRPV1).
Target Subcellular Location
Secreted.
Target Research Area
Signal Transduction
Target Synonyms
Cementoin; ELAF_HUMAN; Elafin; Elafin/Skalp; Elastase Specific Inhibitor; Elastase-specific inhibitor; ES 1; ESI; Peptidase inhibitor 3; Peptidase inhibitor 3; skin derived; PI 3; PI-3; PI3; Pre elafin; Protease inhibitor 3 skin derived ; Protease inhibitor 3; skin derived (SKALP); Protease inhibitor WAP3; SKALP; Skin derived Anti leukoproteinase; Skin-derived antileukoproteinase; Trappin 2; WAP four disulfide core domain 14; WAP four disulfide core domain protein 14; WAP four-disulfide core domain protein 14; WAP3; WFDC14
Target Background
This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.
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