Recombinant Human Matrix metalloproteinase-20 (MMP20)

The plasmid vector incorporates the gene encoding the Human MMP20 protein (108-483aa), generating recombinant plasmid, which is then introduced into baculovirus cells. Selection of positive baculovirus cells is based on their capacity to withstand a particular antibiotic. Subsequently, the baculovirus cells containing the recombinant plasmid are cultivated under conditions that facilitate the expression of the Human MMP20 gene. The protein carries a N-terminal 10xHis tag and C-terminal Myc tag. Following expression, affinity purification is employed to isolate and purify the recombinant Human MMP20 protein from the cell lysate. Denaturing SDS-PAGE is used to resolve the resulting recombinant protein, enabling an estimation of its purity, exceeding 85%.

ACP05039

Number

Cat.No	ACP05039	Target Name	MMP20
Target Synonyms	AI2A2; Enamel metalloproteinase; Enamelysin; Matrix metalloproteinase 20; Matrix metalloproteinase-20; MMP 20; MMP-20; MMP20; MMP20_HUMAN	Form	Liquid or Lyophilized powder
Expression System	Baculovirus	Expression Range	108-483aa
Mol Weight	46.4	Protein Length	Full Length of Mature Protein
Purity	Greater than 85% as determined by SDS-PAGE.	Storage Buffer	5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Cat.No

ACP05039

Target Name

MMP20

Target Synonyms

AI2A2; Enamel metalloproteinase; Enamelysin; Matrix metalloproteinase 20; Matrix metalloproteinase-20; MMP 20; MMP-20; MMP20; MMP20_HUMAN

Form

Liquid or Lyophilized powder

Expression System

Baculovirus

Expression Range

108-483aa

Mol Weight

46.4

Protein Length

Full Length of Mature Protein

Purity

Greater than 85% as determined by SDS-PAGE.

Storage Buffer

5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0.

Target Species	Human	Uniprot ID	O60882

Background Information

Uniprot Id

O60882
Target Species

Human
Target Name

MMP20
Target Full Name

Matrix metalloproteinase-20
Target Function

Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Cleaves aggrecan at the '360-Asn-|-Phe-361' site.
Target Involvement

Amelogenesis imperfecta, hypomaturation type, 2A2 (AI2A2)
Target Subcellular Location

Secreted, extracellular space, extracellular matrix.
Target Protein Families

Peptidase M10A family
Target Tissue Specificity

Expressed specifically in the enamel organ.
Target Research Area

Others
Target Synonyms

AI2A2; Enamel metalloproteinase; Enamelysin; Matrix metalloproteinase 20; Matrix metalloproteinase-20; MMP 20; MMP-20; MMP20; MMP20_HUMAN
Target Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3.

Recombinant Human Matrix metalloproteinase-20 (MMP20)

Specifications

Immunogen Information

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