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The gene encoding the Human GCGR protein (26-136aa) is inserted into a plasmid vector to form the recombinant plasmid, which is introduced into baculovirus cells. baculovirus cells that have successfully taken up the recombinant plasmid can be selected based on their ability to survive in the presence of a specific antibiotic. The recombinant plasmid-containing baculovirus cells are cultured under conditions that promote the expression of the interested gene. The protein is fused with a N-terminal 10xHis tag and C-terminal Myc tag. After expression, the recombinant Human GCGR protein is isolated and purified from the cell lysate through affinity purification. The resulting recombinant Human GCGR protein is resolved by denaturing SDS-PAGE, allowing its purity to be estimated, greater than 85%.
The gene encoding the Human GCGR protein (26-136aa) is inserted into a plasmid vector to form the recombinant plasmid, which is introduced into baculovirus cells. baculovirus cells that have successfully taken up the recombinant plasmid can be selected based on their ability to survive in the presence of a specific antibiotic. The recombinant plasmid-containing baculovirus cells are cultured under conditions that promote the expression of the interested gene. The protein is fused with a N-terminal 10xHis tag and C-terminal Myc tag. After expression, the recombinant Human GCGR protein is isolated and purified from the cell lysate through affinity purification. The resulting recombinant Human GCGR protein is resolved by denaturing SDS-PAGE, allowing its purity to be estimated, greater than 85%.
| Cat.No | ACP05077 | Target Name | GCGR |
|---|---|---|---|
| Target Synonyms | GCGR; Glucagon receptor; GL-R | Form | Liquid or Lyophilized powder |
| Expression System | Baculovirus | Protein Length | Partial |
| Purity | >85% (SDS-PAGE) | Storage Buffer | 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, Liquid form: default storage buffer is Tris/PBS-based buffer, pH 8.0. |
| Target Species | Human | Uniprot ID | P47871 |
|---|
Uniprot Id
P47871
Target Species
Human
Target Name
GCGR
Target Full Name
Glucagon receptor
Target Function
G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.
Target Subcellular Location
Cell membrane; Multi-pass membrane protein.
Target Protein Families
G-protein coupled receptor 2 family
Target Research Area
Cancer
Target Synonyms
GCGR; Glucagon receptor; GL-R
Target Background
The protein encoded by this gene is a glucagon receptor that is important in controlling blood glucose levels. Defects in this gene are a cause of non-insulin-dependent diabetes mellitus (NIDDM).
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