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The recombinant antibody against DHFR was produced using a synthesized peptide derived from human DHFR as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IHC, IP.
The recombinant antibody against DHFR was produced using a synthesized peptide derived from human DHFR as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IHC, IP.
$350.00
Western Blot | Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, Rat heart tissue, Mouse kidney tissue, Mouse liver tissue | All lanes: DHFR antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 22, 16 kDa | Observed band size: 22 kDa
| Cat.No | ADC-56956A | Clonality | Monoclonal |
|---|---|---|---|
| Target Name | DHFR | Target Synonyms | DHFR, Dihydrofolate reductase (EC 1.5.1.3) |
| Form | Liquid | Species Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG | Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline |
| Purification Method | Affinity-chromatography purified | Conjugate | Non-conjugated |
| Application | ELISA, IHC, WB, IP | Storage | Upon receipt |
| Immunogen Description | A synthesized peptide derived from human DHFR | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P00374 |
Uniprot Id
P00374
Target Species
Human
Target Name
DHFR
Target Full Name
Dihydrofolate reductase
Target Function
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.
Target Involvement
Megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD)
Target Subcellular Location
Mitochondrion. Cytoplasm.
Target Protein Families
Dihydrofolate reductase family
Target Tissue Specificity
Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.
Target Research Area
Cell Biology, Signal Transduction
Target Synonyms
DHFR; DHFRP1; Dihydrofolate reductase; DYR; DYR_HUMAN; EC 1.5.1.3
Target Background
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.
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