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The antibody against acnA was raised in rabbit using the Recombinant Escherichia coli (strain K12) acnA protein (2-280) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB.
The antibody against acnA was raised in rabbit using the Recombinant Escherichia coli (strain K12) acnA protein (2-280) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB.
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Western Blot | Positive WB detected in: recombinant protein | All lanes: acnA Antibody at 1:1000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 32 kDa | Observed band size: 32 kDa
| Cat.No | ADC-55376A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | acnA |
| Target Synonyms | Aconitate hydratase A (ACN) (Aconitase) (EC 4.2.1.3) (Iron-responsive protein-like) (IRP-like) (RNA-binding protein) (Stationary phase enzyme) acnA acn b1276 JW1268 | Form | Liquid |
| Species Reactivity | Escherichia coli (strain K12) | Isotype | IgG |
| Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 | Purification Method | >95%, Protein G purified |
| Conjugate | Non-conjugated | Application | ELISA, WB |
| Immunogen Description | Recombinant Escherichia coli (strain K12) acnA protein (2-280) | Target Species | Escherichia coli (strain K12) |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P25516 |
Uniprot Id
P25516
Target Species
E.coli
Target Name
ACNA
Target Function
Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. The apo form of AcnA functions as a RNA-binding regulatory protein which plays a role as a maintenance or survival enzyme during nutritional or oxidative stress. During oxidative stress inactive AcnA apo-enzyme without iron sulfur clusters binds the acnA mRNA 3' UTRs (untranslated regions), stabilizes acnA mRNA and increases AcnA synthesis, thus mediating a post-transcriptional positive autoregulatory switch. AcnA also enhances the stability of the sodA transcript.
Target Protein Families
Aconitase/IPM isomerase family
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