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The antibody against degP was raised in rabbit using the Recombinant Escherichia coli Periplasmic serine endoprotease DegP (degP) (27-474AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Protein A/G purified. This antibody has been validated on ELISA, WB.
The antibody against degP was raised in rabbit using the Recombinant Escherichia coli Periplasmic serine endoprotease DegP (degP) (27-474AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Protein A/G purified. This antibody has been validated on ELISA, WB.
$299.00
Western Blot | Positive WB detected in Recombinant protein | All lanes: degP antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 62.8 kDa | Observed band size: 65 kDa
| Cat.No | ADC-01751A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | degP |
| Form | Liquid | Species Reactivity | Escherichia coli |
| Isotype | IgG | Storage Buffer | 0.01M PBS, 0.03% Proclin 300; Constituents: 50% Glycerol, PH 7.4 |
| Purification Method | Protein A/G purified | Conjugate | Non-conjugated |
| Application | ELISA, WB | Storage | Upon receipt |
| Immunogen Description | Recombinant Escherichia coli Periplasmic serine endoprotease DegP (degP) (27-474AA) | Target Species | Escherichia coli (strain K12) |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P0C0V0 |
Uniprot Id
P0C0V0
Target Species
E.coli
Target Name
DEGP
Target Function
DegP acts as a chaperone at low temperatures but switches to a peptidase (heat shock protein) at higher temperatures. Degrades transiently denatured and unfolded or misfolded proteins which accumulate in the periplasm following heat shock or other stress conditions. DegP is efficient with Val-Xaa and Ile-Xaa peptide bonds, suggesting a preference for beta-branched side chain amino acids. Only unfolded proteins devoid of disulfide bonds appear capable of being cleaved, thereby preventing non-specific proteolysis of folded proteins. Its proteolytic activity is essential for the survival of cells at elevated temperatures. It can degrade IciA, Ada, casein, globin and PapA. DegP shares specificity with DegQ. DegP is also involved in the biogenesis of partially folded outer-membrane proteins (OMP).
Target Subcellular Location
Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
Target Protein Families
Peptidase S1C family
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