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The antibody against AAK1 was raised in rabbit using the Fusion protein corresponding to N terminal 236 amino acids of Human Adaptor-associated protein kinase 1 as the immunogen. This antibody has been validated on ELISA, WB, IHC.
The antibody against AAK1 was raised in rabbit using the Fusion protein corresponding to N terminal 236 amino acids of Human Adaptor-associated protein kinase 1 as the immunogen. This antibody has been validated on ELISA, WB, IHC.
$297.00
Gel: 10%SDS-PAGE Lysate: 30 ug Human fetal brain tissue lysate Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 30 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 31024 (AAK1 Antibody) at dilution 1/30, on the right is treated with the fusion protein.2
| Cat.No | ADC-43042A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | AAK1 |
| Form | Liquid | Species Reactivity | Human, Mouse, Rat |
| Storage Buffer | 0.05% sodium azide and 50% glycerol., pH 7.3, Supplied at 1.1mg/mL in phosphate buffered saline (without Mg2+ and Ca2+) | Conjugate | Non-conjugated |
| Application | ELISA, IHC, WB | Storage | Upon receipt |
| Immunogen Description | Fusion protein corresponding to N terminal 236 amino acids of Human Adaptor-associated protein kinase 1 | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | Q2M2I8 |
Uniprot Id
Q2M2I8
Target Species
Human
Target Name
AAK1
Target Full Name
AP2-associated protein kinase 1
Target Function
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.; (Microbial infection) By regulating clathrin-mediated endocytosis, AAK1 plays a role in the entry of hepatitis C virus as well as for the lifecycle of other viruses such as Ebola and Dengue.
Target Subcellular Location
Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Cell junction, synapse, presynapse.
Target Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Target Tissue Specificity
Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.
Target Synonyms
AAK 1; Aak1; AAK1_HUMAN; Adaptor associated kinase 1; Adaptor-associated kinase 1; AP2 associated kinase 1; AP2 associated protein kinase 1; AP2-associated protein kinase 1; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; KIAA1048; MGC138170; MGC164568; MGC164570
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