Rabbit anti-Human AKT1/AKT2/AKT3 Polyclonal Antibody

The antibody against AKT1/AKT2/AKT3 was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 1-123 of human AKT1/AKT2/AKT3 (NP_005154.2) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, ELISA.

ADA-10665A

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Western blot analysis of various lysates, using AKT1/AKT2/AKT3 Rabbit pAb at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Specifications

Cat.No	ADA-10665A	Clonality	Polyclonal
Host Species	Rabbit	Target Name	AKT1/AKT2/AKT3
Form	Liquid	Species Reactivity	Human, Mouse, Rat
Isotype	IgG	Storage Buffer	50% Glycerol, PBS with 0.05% proclin300, pH7.3.
Purification Method	Affinity purification	Positive Samples	C6, HeLa, NIH/3T3, Rat brain, MCF7, Mouse brain, SH-SY5Y
Application	ELISA, WB

Immunogen Information

Immunogen Description	Recombinant fusion protein containing a sequence corresponding to amino acids 1-123 of human AKT1/AKT2/AKT3 (NP_005154.2).	Target Species	Human
Immunogen Sequence	MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSG	Uniprot ID	P31749, P31751, Q9Y243

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