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The antibody against APAF1 was raised in rabbit using the Synthesized peptide derived from the C-terminal region of Human Apaf-1-ALT. as the immunogen. This antibody exists as a non-conjugated isotype IgG. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on WB, IHC, IF, ELISA.
The antibody against APAF1 was raised in rabbit using the Synthesized peptide derived from the C-terminal region of Human Apaf-1-ALT. as the immunogen. This antibody exists as a non-conjugated isotype IgG. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on WB, IHC, IF, ELISA.
$167.00
Western Blot analysis of COLO205 cells using Apaf-1-ALT Polyclonal Antibody
Western Blot analysis of Jurkat cells using Apaf-1-ALT Polyclonal Antibody2
| Cat.No | ADC-39321A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | APAF1 |
| Form | Liquid | Species Reactivity | Human |
| Isotype | IgG | Storage Buffer | 0.5% BSA and 0.02% sodium azide., Liquid in PBS containing 50% glycerol |
| Purification Method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | Conjugate | Non-conjugated |
| Application | ELISA, IF, IHC, WB | Storage | Upon receipt |
| Immunogen Description | Synthesized peptide derived from the C-terminal region of Human Apaf-1-ALT. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | O14727-6 |
Uniprot Id
O14727
Target Species
Human
Target Name
APAF1
Target Full Name
Apoptotic protease-activating factor 1
Target Function
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Target Subcellular Location
Cytoplasm.
Target Tissue Specificity
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Target Research Area
Cancer
Target Synonyms
APAF 1; Apaf-1; APAF_HUMAN; Apaf1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor 1; Apoptotic protease activating factor; Apoptotic protease-activating factor 1; CED 4; CED4; KIAA0413
Target Background
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
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