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The antibody against CA5A was raised in rabbit using the Synthesized peptide derived from internal of Human CA5A. as the immunogen. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on ELISA, WB.
The antibody against CA5A was raised in rabbit using the Synthesized peptide derived from internal of Human CA5A. as the immunogen. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody has been validated on ELISA, WB.
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Western blot analysis of extracts from A549 cells, LOVO cells and K562 cells, using CA5A antibody.
| Cat.No | ADC-41369A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | CA5A |
| Target Synonyms | CA5A antibody; CA5Carbonic anhydrase 5A antibody; mitochondrial antibody; EC 4.2.1.1 antibody; Carbonate dehydratase VA antibody; Carbonic anhydrase VA antibody; CA-VA antibody | Form | Liquid |
| Species Reactivity | Human | Storage Buffer | PH 7.4, 0.02% sodium azide and 50% glycerol., 150mM NaCl, Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) |
| Purification Method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | Application | ELISA, WB |
| Storage | Upon receipt |
| Immunogen Description | Synthesized peptide derived from internal of Human CA5A. | Target Species | Human |
|---|---|---|---|
| Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. | Uniprot ID | P35218 |
Uniprot Id
P35218
Target Species
Human
Target Name
CA5A
Target Full Name
Carbonic anhydrase 5A, mitochondrial
Target Function
Reversible hydration of carbon dioxide. Low activity.
Target Involvement
Hyperammonemia due to carbonic anhydrase VA deficiency (CA5AD)
Target Subcellular Location
Mitochondrion.
Target Protein Families
Alpha-carbonic anhydrase family
Target Research Area
Metabolism
Target Synonyms
CA5A; CA5Carbonic anhydrase 5A; mitochondrial; EC 4.2.1.1; Carbonate dehydratase VA; Carbonic anhydrase VA; CA-VA
Target Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VA is localized in the mitochondria and expressed primarily in the liver. It may play an important role in ureagenesis and gluconeogenesis. CA5A gene maps to chromosome 16q24.3 and an unprocessed pseudogene has been assigned to 16p12-p11.2.
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