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The antibody against CPT1B was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human CPT1B (NP_004368.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
The antibody against CPT1B was raised in Rabbit using the recombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human CPT1B (NP_004368.1) as the immunogen. The polyclonal antibody exists as a isotype IgG, by affinity purification. This antibody has been validated on WB, IHC-P, ELISA.
Western blot analysis of lysates from Rat skeletal muscle, using CPT1B Rabbit pAb at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
| Cat.No | ADA-12818A | Clonality | Polyclonal |
|---|---|---|---|
| Host Species | Rabbit | Target Name | CPT1B |
| Target Synonyms | CPTI; CPT1M; MCPT1; CPT1-M; CPTI-M; M-CPT1; MCCPT1; CPT1B | Form | Liquid |
| Species Reactivity | Human, Mouse, Rat | Isotype | IgG |
| Storage Buffer | 50% Glycerol, PBS with 0.02% sodium azide, pH7.3. | Purification Method | Affinity purification |
| Positive Samples | Rat skeletal muscle | Application | ELISA, WB, IHC-P |
| Immunogen Description | Recombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human CPT1B (NP_004368.1). | Target Species | Human |
|---|---|---|---|
| Uniprot ID | Q92523 | Immunogen Sequence |
Uniprot Id
Q92523
Target Species
Human
Target Name
CPT1B
Target Full Name
Carnitine O-palmitoyltransferase 1, muscle isoform
Target Subcellular Location
Mitochondrion outer membrane; Multi-pass membrane protein.
Target Protein Families
Carnitine/choline acetyltransferase family
Target Tissue Specificity
Strong expression in heart and skeletal muscle. No expression in liver and kidney.
Target Synonyms
CPT1B; KIAA1670; Carnitine O-palmitoyltransferase 1, muscle isoform; CPT1-M; Carnitine O-palmitoyltransferase I, muscle isoform; CPT I; CPTI-M; Carnitine palmitoyltransferase 1B; Carnitine palmitoyltransferase I-like protein
Target Background
The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene.
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